A yeast homolog of chromatin assembly factor 1 is involved in early ribosome assembly
Identifieur interne : 000234 ( France/Analysis ); précédent : 000233; suivant : 000235A yeast homolog of chromatin assembly factor 1 is involved in early ribosome assembly
Auteurs : Sigrid Schaper [Allemagne, France] ; Micheline Fromont-Racine [France] ; Patrick Linder [Suisse] ; Jesús De La Cruz [Suisse] ; Abdelkader Namane [France] ; Moshe Yaniv [France]Source :
- Current Biology [ 0960-9822 ] ; 2001.
English descriptors
- Teeft :
- Brief communication, Cell biol, Chromatin assembly factor, Cleavage site, Control reactions, Copurifying, Current biology, Degradation products, Different regions, Docteur roux, Early precursor rrna copurifies, Final eluate, Free couples, Hybridization, Institut pasteur, Mass spectrometry, Mass spectrometry analysis, Nylon membrane, Oligonucleotides number, Parallel preparation, Paris cedex, Polysome analysis, Polysome profile, Polysomes, Precursor, Precursor rrna, Preribosomal particle, Primer, Primer hybridization assay, Primer number, Primers number, Protein synthesis, Proteins copurify, Ribonuclease, Ribonuclease inhibitor, Ribosomal, Ribosomal protein, Ribosomal proteins, Ribosomal subunit, Ribosomal subunit assembly, Ribosomal subunits, Ribosome, Ribosome assembly, Ribosome biogenesis, Rrb1p, Rrb1p function results, Rrna, Saccharomyces cerevisiae, Same membrane, Spectrometry, Substoichiometrically copurifying, Subunit, Sucrose gradients, Supplementary material, Unpublished data, Yeast, Yeast homolog.
Abstract
Abstract: Cells have a recurrent need for the correct assembly of protein-nucleic acid complexes. We have studied a yeast homolog of the smallest subunit of chromatin assembly factor 1 (CAF1), encoded by YMR131c and termed “RRB1” [1]. Unlike other yeast homologs, Msi1p, and Hat2p, Rrb1p is essential for cell viability. Impairment of Rrb1p function results in decreased levels of free 60S ribosomal subunits and the appearance of half-mer polysomes, suggesting its involvement in ribosome biogenesis. Using tandem affinity purification (TAP [2]) combined with mass spectrometry, we show that Rrb1p is associated with ribosomal protein L3. A fraction of Rrb1p is also found in a protein-precursor rRNA complex containing at least ten other early-assembling ribosomal proteins. We propose that Rrb1p is required for proper assembly of preribosomal particles during early ribosome biogenesis, presumably by targeting L3 onto the 35S precursor rRNA. This action may resemble the mechanism by which CAF1 assembles histones H3/H4 onto newly replicated DNA.
Url:
DOI: 10.1016/S0960-9822(01)00584-X
Affiliations:
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ISTEX:947E6E140DBABF8D8A3368FF8013F30F08DB0153Le document en format XML
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communication</term><term>Cell biol</term><term>Chromatin assembly factor</term><term>Cleavage site</term><term>Control reactions</term><term>Copurifying</term><term>Current biology</term><term>Degradation products</term><term>Different regions</term><term>Docteur roux</term><term>Early precursor rrna copurifies</term><term>Final eluate</term><term>Free couples</term><term>Hybridization</term><term>Institut pasteur</term><term>Mass spectrometry</term><term>Mass spectrometry analysis</term><term>Nylon membrane</term><term>Oligonucleotides number</term><term>Parallel preparation</term><term>Paris cedex</term><term>Polysome analysis</term><term>Polysome profile</term><term>Polysomes</term><term>Precursor</term><term>Precursor rrna</term><term>Preribosomal particle</term><term>Primer</term><term>Primer hybridization assay</term><term>Primer number</term><term>Primers number</term><term>Protein synthesis</term><term>Proteins copurify</term><term>Ribonuclease</term><term>Ribonuclease inhibitor</term><term>Ribosomal</term><term>Ribosomal protein</term><term>Ribosomal proteins</term><term>Ribosomal subunit</term><term>Ribosomal subunit assembly</term><term>Ribosomal subunits</term><term>Ribosome</term><term>Ribosome assembly</term><term>Ribosome biogenesis</term><term>Rrb1p</term><term>Rrb1p function results</term><term>Rrna</term><term>Saccharomyces cerevisiae</term><term>Same membrane</term><term>Spectrometry</term><term>Substoichiometrically copurifying</term><term>Subunit</term><term>Sucrose gradients</term><term>Supplementary material</term><term>Unpublished data</term><term>Yeast</term><term>Yeast homolog</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Cells have a recurrent need for the correct assembly of protein-nucleic acid complexes. We have studied a yeast homolog of the smallest subunit of chromatin assembly factor 1 (CAF1), encoded by YMR131c and termed “RRB1” [1]. Unlike other yeast homologs, Msi1p, and Hat2p, Rrb1p is essential for cell viability. Impairment of Rrb1p function results in decreased levels of free 60S ribosomal subunits and the appearance of half-mer polysomes, suggesting its involvement in ribosome biogenesis. Using tandem affinity purification (TAP [2]) combined with mass spectrometry, we show that Rrb1p is associated with ribosomal protein L3. A fraction of Rrb1p is also found in a protein-precursor rRNA complex containing at least ten other early-assembling ribosomal proteins. We propose that Rrb1p is required for proper assembly of preribosomal particles during early ribosome biogenesis, presumably by targeting L3 onto the 35S precursor rRNA. This action may resemble the mechanism by which CAF1 assembles histones H3/H4 onto newly replicated DNA.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>France</li><li>Suisse</li></country><region><li>Île-de-France</li></region><settlement><li>Paris</li></settlement></list><tree><country name="Allemagne"><noRegion><name sortKey="Schaper, Sigrid" sort="Schaper, Sigrid" uniqKey="Schaper S" first="Sigrid" last="Schaper">Sigrid Schaper</name></noRegion></country><country name="France"><region name="Île-de-France"><name sortKey="Schaper, Sigrid" sort="Schaper, Sigrid" uniqKey="Schaper S" first="Sigrid" last="Schaper">Sigrid Schaper</name></region><name sortKey="Fromont Racine, Micheline" sort="Fromont Racine, Micheline" uniqKey="Fromont Racine M" first="Micheline" last="Fromont-Racine">Micheline Fromont-Racine</name><name sortKey="Namane, Abdelkader" sort="Namane, Abdelkader" uniqKey="Namane A" first="Abdelkader" last="Namane">Abdelkader Namane</name><name sortKey="Yaniv, Moshe" sort="Yaniv, Moshe" uniqKey="Yaniv M" first="Moshe" last="Yaniv">Moshe Yaniv</name></country><country name="Suisse"><noRegion><name sortKey="Linder, Patrick" sort="Linder, Patrick" uniqKey="Linder P" first="Patrick" last="Linder">Patrick Linder</name></noRegion><name sortKey="De La Cruz, Jesus" sort="De La Cruz, Jesus" uniqKey="De La Cruz J" first="Jesús" last="De La Cruz">Jesús De La Cruz</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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